cell mouse anti pig cd3 fitc bio rad mca5951f ppt3  (Bio-Rad)

Journal: The Journal of Immunology Author Choice

Article Title: Simultaneous Aerosol and Intramuscular Immunization with Influenza Vaccine Induces Powerful Protective Local T Cell and Systemic Antibody Immune Responses in Pigs

doi: 10.4049/jimmunol.2001086

Figure Lengend Snippet: List of Abs used for intracellular cytokine staining and NP tetramers enumeration

Article Snippet: The lymphocytes were then stained with surface markers , including anti-porcine CD3-FITC (clone PPT3; Bio-Rad Laboratories).

Techniques: Staining

Journal: The Journal of Immunology Author Choice

Article Title: Simultaneous Aerosol and Intramuscular Immunization with Influenza Vaccine Induces Powerful Protective Local T Cell and Systemic Antibody Immune Responses in Pigs

doi: 10.4049/jimmunol.2001086

Figure Lengend Snippet: Experimental design and viral load in nasal swabs and BAL. ( A ) Babraham pigs were immunized with S-FLU by Aer, i.m., or simultaneously by Aer and i.m. (SIM) and boosted 3 wk apart. Control animals were left untreated. All animals were challenged with H1N1pdm09 virus 3 wk after the boost. Swabs were taken daily postchallenge, and all pigs were culled 4 DPC. Half of the pigs were infused intravenously with anti-porcine CD3 mAb 10 min prior to sacrifice. ( B ) Virus titer in nasal swabs measured by plaque assay at one, two, three, and 4 DPC. ( C ) Area under the curve (AUC) of viral titer in the nasal swabs over time. ( D ) Viral titer in the BAL 4 DPC. The data represent the average of two separate assays; each data points indicates an individual animal and the horizontal line the mean of the group. Data were analyzed using the Kruskal–Wallis test. Asterisks indicate a significant difference from the control group: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The lymphocytes were then stained with surface markers , including anti-porcine CD3-FITC (clone PPT3; Bio-Rad Laboratories).

Techniques: Plaque Assay

Journal: The Journal of Immunology Author Choice

Article Title: Simultaneous Aerosol and Intramuscular Immunization with Influenza Vaccine Induces Powerful Protective Local T Cell and Systemic Antibody Immune Responses in Pigs

doi: 10.4049/jimmunol.2001086

Figure Lengend Snippet: Porcine tissue-resident memory cells. Three pigs from the immunized groups and two control pigs were infused i.v. with CD3 mAb and culled 10 min later. Lymphocytes were isolated and stained ex vivo with the same clone of CD3 Ab labeled with FITC as described in the . As the infused CD3 does not saturate all CD3 sites, some nasal turbinate, tracheal, and lung tissue T cells are double positive (intravascular cells). A proportion of BAL, nasal turbinate, and tracheal cells are unstained by intravascular mAb, indicating tissue residency (top row). The top row shows representative FACS plots for each tissue of infused animal. The bottom row shows staining in animals not infused with CD3 mAb and stained ex vivo. The reported proportion of CD3 ex vivo + CD3 i.v. − and CD3 ex vivo + CD3 i.v. + are calculated from total CD3 + .

Article Snippet: The lymphocytes were then stained with surface markers , including anti-porcine CD3-FITC (clone PPT3; Bio-Rad Laboratories).

Techniques: Isolation, Staining, Ex Vivo, Labeling